Journal: The Journal of Neuroscience
Article Title: Target-Specific Neuropeptide Y-Ergic Synaptic Inhibition and Its Network Consequences within the Mammalian Thalamus
doi: 10.1523/JNEUROSCI.23-29-09639.2003
Figure Lengend Snippet: Exogenous and endogenous NPY receptor activation suppresses thalamic network oscillations. A, Contour plot (A1) and representative multiunit recordings (A2) showing effects of NPY perfusion on intrathalamic rhythmic oscillations in RT of a rat thalamic slice exposed to bicuculline (10 μm). A1, Contour plot representing ratemeter of extracellular spikes. The left vertical bar shows time of perfusion with control solution (open bar), NPY (black bar), and after washout (gray bar). The x-axis represents time within each evoked oscillation. The y-axis represents the time course throughout the experiment (i.e., Pre-drug, NPY, Wash). The z-axis represents the spike intensity during a single evoked oscillation; warmer color levels correspond to a higher frequency of firing. A2, Each trace shows oscillatory burst responses to a single extracellular stimulus in control solution (bottom, black trace), during a 200 nm NPY application (middle, gray), and 10 min after drug washout (top, black). The vertical dashed lines indicate timing of late recurrent bursts in control conditions. The gray arrows point to bursts in NPY that are delayed and reduced in number per response compared with controls. B1, B2, Contour plot (B1) and extracellular multiunit recordings of oscillatory activity (B2) in control solution (bottom), during 20 nm BIBP3226 application (middle), and after washout (top) in another rat thalamic slice. The vertical dashed lines indicate the time at which bursts occurred in control conditions (bottom). BIBP3226 speeds the oscillation and increases the number of multiunit bursts in the oscillatory response (gray arrows in middle trace). C, Autocorrelograms of the experiments of A1 and B1 showing decreased overall activity (amplitude of central peak) and synchrony (decreased peak to valley ratio) during application of NPY (thin arrow) and increased activity and synchrony during application of BIBP3226 (thick line arrow). Black curve, Control. D, Summary of the effects of NPY (gray bars; n = 7) and BIBP3226 (black bars; n = 11) on network oscillations. D1, The number of spikes per episode (spike count), normalized against controls. D2, Time constant (τ) of the decay of the oscillation (Huntsman et al., 1999) normalized against controls. *p < 0.05; **p < 0.01 for drug condition versus control.
Article Snippet: D2 , Time constant (τ) of the decay of the oscillation ( Huntsman et al., 1999 ) normalized against controls.
Techniques: Activation Assay, Control, Activity Assay
